Utilization of two distinct modes of replication by a hybrid plasmid constructed in vitro from separate replicons.
نویسندگان
چکیده
A hybrid plasmid, pSC134, that codes for two distinct sets of replication functions has been constructed in vitro by ligation of EcoRI endonuclease-cleaved pSC101 and Col E1 plasmid replicons, and has been introduced into Escherichia coli by transformation. The replication properties of the pSC134 plasmid in DNA polymerase I-defective mutants or in the presence of chloramphenicol indicate that this hybrid plasmid can utilize the functionally distinct modes of replication specified by both of its parent replicons.
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عنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 71 11 شماره
صفحات -
تاریخ انتشار 1974